Cross-React : Prediction of allergen cross-reactivity

For cross-reactivity prediction, please select a group of amino acids located in the epitope region and upload a PDB file of interest. The server will use this information to search all allergenic protein structures listed in the Structural Database of Allergenic Proteins (SDAP). Please do not upload a PDB complex. Use only one PDB chain name or a monomeric protein structure. You may use a PDB ID or a single word as the filename for your PDB file. Average processing time per query is approximately 90 seconds. Latest release: Version 2.0 — includes minor bug fixes from Version 1. Users can now search more than 1,500 allergen structures available in the SDAP database for cross-reactivity.

Reference:
Negi SS and Braun W, Cross-React: a new structural bioinformatics method for predicting allergen cross-reactivity. Bioinformatics 33 (7): 1014-1020, 2017. pdf

Note: If you have a protein complex, then list of amino acids in the binding site can be calculated from https://curie.utmb.edu/pdbcomplex.html or https://curie.utmb.edu/usercomplex.html utility.

🧬 Cross-React

List of amino acids in the binding site: e.g., 42, 43, 44, 45, 46, 47, 48, 49, 50, 51
(For conformational epitopes, please use only surface-exposed residues.)

Project name:
Email Address: For information purpose only
Patch size (A):
Area cutoff (A^2):
Correlation cutoff: e.g 0.8
PDB file name:

How to use the webserver: Please see below a screenshot for the cross-react analysis of Bet v 1.

Example input from the WebServer.

Example output from the WebServer.

Cross-React: Prediction of allergen cross-reactivity

🧬 Cross-React